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DFG SFB 604
DFG SFB 604
DFG SFB 604: Multifunctional Signalling Proteins
Homepage of the SFB 604
The concept of unidirectional intracellular signalling paths controlling defined cellular functions dominated molecular cell biology until the beginning of this century. Experimental results of the recent years corroded this idea disclosing multifunctional features of signalling proteins. Inhibitory analysis and transfection studies disclosed differential signalling reactions of these proteins in dependence of the cell type. The question arouse, how one signalling protein is able to control one cell function in one cell type and a different one in another cell. This issue, which is tightly connected to the understanding of "signalling networks", "cross talks", "signalosomes" and other poorly defined keywords, is the main objective of the Collaborative Research Centre (Sonderforschungsbereich") SFB 604.
The SFB is divided into project areas A and B, which are addressing the interdependence of signalling reactions and cell functions from two angles.
Project area A "From cells to molecules" aims to analyse the complex interactions of signalling proteins in selected cell lines and primary tissue. Besides classical inhibitory analysis, protein-protein-interactions of the signalling proteins and corresponding formation and dissociation of signalling protein complexes are under investigation. All molecular parameters are explored in correlation to the functional pattern of the cell.
Projects in area B "From molecules to cells" are focussing on the biochemical characterisation of signalling proteins as an initial approach. Molecular interactions and enzymatic functions of these proteins are investigated in detail and mutational approaches are employed to dissect sub-functions of the protein. Following the heterologous expression of wild- type protein, protein variants and the knockdown of protein by RNAi in selected cell lines the regulatory functions of these proteins are examined.
Projects in area C in the current funding period aim to explore specific techniques essential for the experimental work in the SFB. Thus, advanced methods for the isolation and characterisation of signalling protein complexes have been developed and attempts have been started to decipher the architecture of cell-specific signalling complexes on the basis of genomic data.
Homepage of the SFB 604
The concept of unidirectional intracellular signalling paths controlling defined cellular functions dominated molecular cell biology until the beginning of this century. Experimental results of the recent years corroded this idea disclosing multifunctional features of signalling proteins. Inhibitory analysis and transfection studies disclosed differential signalling reactions of these proteins in dependence of the cell type. The question arouse, how one signalling protein is able to control one cell function in one cell type and a different one in another cell. This issue, which is tightly connected to the understanding of "signalling networks", "cross talks", "signalosomes" and other poorly defined keywords, is the main objective of the Collaborative Research Centre (Sonderforschungsbereich") SFB 604.
The SFB is divided into project areas A and B, which are addressing the interdependence of signalling reactions and cell functions from two angles.
Project area A "From cells to molecules" aims to analyse the complex interactions of signalling proteins in selected cell lines and primary tissue. Besides classical inhibitory analysis, protein-protein-interactions of the signalling proteins and corresponding formation and dissociation of signalling protein complexes are under investigation. All molecular parameters are explored in correlation to the functional pattern of the cell.
Projects in area B "From molecules to cells" are focussing on the biochemical characterisation of signalling proteins as an initial approach. Molecular interactions and enzymatic functions of these proteins are investigated in detail and mutational approaches are employed to dissect sub-functions of the protein. Following the heterologous expression of wild- type protein, protein variants and the knockdown of protein by RNAi in selected cell lines the regulatory functions of these proteins are examined.
Projects in area C in the current funding period aim to explore specific techniques essential for the experimental work in the SFB. Thus, advanced methods for the isolation and characterisation of signalling protein complexes have been developed and attempts have been started to decipher the architecture of cell-specific signalling complexes on the basis of genomic data.
Homepage of the SFB 604
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